Vitamin D Status Modulates Rat Colonic M3 Muscarinic Receptor Characteristics and Coupling to Guanylate Cyclase

Sharad Khare; Xiao-Ying Tien; David M. Wilson; Ramesh K. Wali; Bruce M. Bissonette; Michael D Sitrin; Thomas A Brasitus

Vitamin D Status Modulates Rat Colonic M3 Muscarinic Receptor Characteristics and Coupling to Guanylate Cyclase

Sharad Khare
, Xiao-Ying Tien
, David M. Wilson
, Ramesh K. Wali
, Bruce M. Bissonette
, Michael D Sitrin
, Thomas A Brasitus

Parkland College

Research output: Contribution to journal › Article › peer-review

Abstract

The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors ( B max) were significantly lower, and dissociation constants ( K d) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

Original language	American English
Journal	FEBS Letters
Volume	347
State	Published - Jun 1994

Disciplines

Biochemistry
Medicine and Health Sciences

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https://febs.onlinelibrary.wiley.com/doi/epdf/10.1016/0014-5793%2894%2900498-6License: Unspecified

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@article{80a5ff058d2e4e1aa2d58792a4cc3dd2,

title = "Vitamin D Status Modulates Rat Colonic M3 Muscarinic Receptor Characteristics and Coupling to Guanylate Cyclase",

abstract = " The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors ( B max) were significantly lower, and dissociation constants ( K d) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.",

author = "Sharad Khare and Xiao-Ying Tien and Wilson, \{David M.\} and Wali, \{Ramesh K.\} and Bissonette, \{Bruce M.\} and Sitrin, \{Michael D\} and Brasitus, \{Thomas A\}",

year = "1994",

month = jun,

language = "American English",

volume = "347",

journal = "FEBS Letters",

issn = "1873-3468 ",

publisher = "John Wiley \& Sons, Inc.",

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T1 - Vitamin D Status Modulates Rat Colonic M3 Muscarinic Receptor Characteristics and Coupling to Guanylate Cyclase

AU - Khare, Sharad

AU - Tien, Xiao-Ying

AU - Wilson, David M.

AU - Wali, Ramesh K.

AU - Bissonette, Bruce M.

AU - Sitrin, Michael D

AU - Brasitus, Thomas A

PY - 1994/6

Y1 - 1994/6

N2 - The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors ( B max) were significantly lower, and dissociation constants ( K d) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

AB - The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors ( B max) were significantly lower, and dissociation constants ( K d) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

UR - http://onlinelibrary.wiley.com/doi/10.1016/0014-5793(94)00498-6/epdf

M3 - Article

SN - 1873-3468

VL - 347

JO - FEBS Letters

JF - FEBS Letters

ER -

Vitamin D Status Modulates Rat Colonic M3 Muscarinic Receptor Characteristics and Coupling to Guanylate Cyclase

Abstract

Disciplines

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